甲基亚硝基脲引起大鼠大肠肿瘤中p53基因突变

解剖学报 2000年第0期第31卷 论著

作者：李佳慧　高爱玲　付松滨　李璞　刘志诚

单位：李佳慧(中国医学科学院 中国协和医科大学实验动物研究所，北京100021)；高爱玲(临汾地区人民医院，临汾041000)；付松滨 李璞 刘志诚(哈尔滨医科大学，哈尔滨150086)

关键词：甲基亚硝基脲；大肠肿瘤；p53基因突变

　　【摘要】目的　检测甲基亚硝基脲诱导大鼠大肠肿瘤中抑癌基因p 53变化 。　方法　应用PCR，SSCP和SSCP和DNA测序的方法。　结果　经PCR-SSCP分析，可见甲基亚硝 基脲诱导大鼠大肠肿瘤中，出现p53基因单链的移位条带，且在第VII外显子；进一步 做双链直 接测序分析，见到p53蛋白编码序列的第244密码子的第3碱基C缺失。　结论 　实验结果提 示大鼠暴露于致癌物甲基亚硝基脲后，产生了大肠肿瘤，且在分子水平上见到了抑癌基因p5 3突变。

　　【中图分类号】Q754；Q95-33　【文献标识码】A　【文章编号】0529-1356(2000)增-97

RAT P53GENE MUTATIONS IN THE COLORECTAL TUMORS INDUCED BY N-METHYL-N-NITROSOUREA

LI Jia-hui

　　(Institute of Laboratory Animal,CAMS and PUMC,Beijing 100021，China;)

　　GAO Ai-ling

　　(the People's Hospital,Linfen 04100,China;)

　　FU Song-bin,LI Pu,LIU Zhi-cheng

　　(Harbin Medical University,Harbin 150086,China)

　　【Abstract】Objective We have examined tumor suppressor gen e p53 from N-methy1-N-nitrosourea(MNU)induced the colorectal tumors in rats. Methods PCR,SSCP and DNA sequence analysis.Results In c olorectal tumors induced by MNU,the mobility shifts of p53 gene mutation w ere obse rved,p53 gene mutation was a deletion at third position C of codon 244 in exon7. Conclusion The present study suggested that MNU induces rat p53 mutations in the colorectal tumors.

　　【Key words】MNU; Colorectal tumors; p53gnen mutation

　　【基金项目】黑龙江省自然科学基金资助项目(94016)

　　【作者简介】李佳慧(1961-)，女(汉族)，辽宁省丹东市人，博士后

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【收稿日期】2000-07-17　【修回日期】2000-08-24